Viruses, bacteria, parasites, fungi, and algae – a layperson's view

 

Introduction

During this extraordinary period of COVID-19 pandemic, the media use the word virus endlessly every day. Among others, the media remind the viewers repetitively how long the virus might remain active on various surfaces. So, the layperson asks: Is a virus a living organism? What is the size of a virus? How are viruses different from bacteria, or parasites, or fungi, or algae? These and similar questions cross a layperson's mind but are not answered by the media.

This article attempts to address some of these questions. It is not a comprehensive narrative on the subject. Far from it, this article only touches upon a few of the relevant terminologies and tries to demystify those for the layperson. The author wishes to disclose here that he is not a medical doctor, or virologist, or microbiologist.  He is a layperson who delved into the subject to satiate his curiosity. He searched reliable sources and collated simple answers to some of the questions and thought it might be of interest to other laypersons.

The naming of pathogens and diseases

Any virus, bacterium, fungus, or another microorganism that can cause a disease is a pathogen. A pathogen may also be referred to as an infectious agent, or merely a germ. (However, this germ is not to be confused with the word germ that is used concerning plants). The term pathogen came into use in the 1880s. A pathogen becomes pathogenic when it has reached a cluster size that is large enough to cause disease.

A pathogen and the associated disease do not necessarily have the same name. Today, the two most used words are COVID-19 and coronavirus. While COVID-19 is the name of the disease, the virus causing the disease is not named 'coronavirus'. Because there are many different types of coronaviruses, many of which cause disease in mammals and birds. The type of coronavirus that causes COVID-19 is called SARS-CoV-2, which stands for severe acute respiratory syndrome coronavirus 2. This name is appropriate because the virus is genetically related to an earlier type of coronavirus that caused the SARS outbreak of 2003; that virus was then named SARS-CoV.  While these two types of coronaviruses belong to the same family, they are nonetheless different. Hence, they have two separate names.

There are different processes, and purposes, for naming viruses and diseases. Viruses are named based on their genetic structure. This facilitates the development of diagnostic tests, vaccines, and medicines. Virologists and the broader scientific community do this work. Viruses are named by the International Committee on Taxonomy of Viruses (ICTV).  (Taxonomy is the science of naming, describing, and classifying organisms and includes all plants, animals, and microorganisms of the world). Diseases are named to enable discussion on disease prevention, spread, transmissibility, and treatment. Human disease preparedness and response is WHO's role. So diseases are named by WHO in the International Classification of Diseases (ICD) (World Health Organization, 2020).

This article deals with some of the pathogens that cause diseases only in human beings, and not in plants, animals, birds, or any non-human living entity.

Viruses

A virus is an infectious agent of small size and simple composition that can multiply only in living cells of humans, animals, plants, and bacteria as they do not have their own mechanism to multiply. When some disease-causing viruses enter host cells, they start making new copies of themselves very quickly. They often outpace the immune system's production of protective antibodies. Rapid virus multiplication can result in cell death and the spread of the virus to nearby cells.

Viruses are only active while intracellular, i.e., they are inside host cells, taking control of those cells' mechanisms and stealing their energy. In contrast, bacteria and parasites can live on the surface of the host's body or inside the host's organs and tissues. Reproduction is another significant difference between viruses and bacteria and parasites. Viruses can multiply inside host cells but cannot reproduce while bacteria and parasites can reproduce. Thus, viruses are not parasites.

Viruses occupy a unique taxonomic position. They are not plants, animals, or bacteria, and they occupy their separate kingdom. Viruses should not even be considered organisms in the strictest sense because they are not free-living. They cannot reproduce and carry on metabolic processes without a host cell. All viruses contain nucleic acid and protein. The nucleic acid can be either DNA (deoxyribonucleic acid) or RNA (ribonucleic acid). The nucleic acid encodes the genetic information unique for each virus (Wagner, 2020). In SARS-CoV-2, for example, the nucleic acid is RNA (Figure 1). Coronavirus is named so because the spike proteins give it the look of a crown or corona.

Figure 1 SARS-CoV-2, courtesy Encyclopaedia Britannica / Patrick O'Neill Riley

As for their size, most viruses vary in diameter from 20 nm (nm = nanometre = 10-9 metre, or one billionth of a metre) to 300 nm. SARS-CoV-2 virus that causes COVID-19 is 60-140 nm in size (Bing.com/images). It is roughly 1000 times smaller than the width of a strand of human hair. Electron microscopy is still at the forefront of clinical diagnoses of viruses. In 1939, Ernst Ruska (Nobel Prize 1986) and his colleagues were the first to visualize viruses (tobacco mosaic virus) (Goldsmith, 2009). So the first visualization of viruses is no more than 81 years old. 

Recently, during COVID-19 attack, in India, the first laboratory-confirmed infection by SARS-CoV-2 was on January 30, 2020. The throat swab specimen had tested positive for SARS-CoV-2 nucleic acid by reverse transmission polymerase chain reaction (RT-PCR) test. The test used transmission electron microscopy (TEM) (Prasad S, 2020). A TEM costs approximately Rs.10 million. However, the PCR test uses a PCR machine. It is a modern high-throughput thermal cycler (also known as a thermocycler, or DNA amplifier). It is a laboratory apparatus that is used commonly to amplify segments of DNA via the polymerase chain reaction (PCR). PCR multiply the virus to millions after which a simple chemical reaction is used to visualize it. Real time PCR test quantitates the virus.

Viral infections and treatments 

Some of the common viral diseases are the common cold, smallpox, rabies, infectious hepatitis, measles, poliomyelitis, influenza, viral encephalitis, mumps, herpes simplex, viral conjunctivitis, Ebola, SARS, and the latest COVID-19.

As there is no medicine to treat most of the viral diseases, the first step in treating a viral infection is preventing its occurrence and spread. Vaccines are available to prevent some common viral infections, such as chickenpox, shingles, influenza, HPV, hepatitis B, hepatitis A, measles, and mumps. The feverish race to discover vaccines for COVID-19 is presently ongoing. Treatment of viral infections is symptomatic and generally includes rest, increased fluids, good nutrition, and may require hospitalization and intensive care, mostly if complications occur. Antibiotics treat bacterial infections and are ineffective for the treatment of viral infections. Sometimes drugs called antiviral drugs may be used to help treat certain types of viral infections. Most antiviral drugs can only help to minimize the severity of a viral infection but cannot cure the infection (Diagnosis, 2015). However, there are a few antiviral drugs that can cure the disease as in hepatitis C.

 Bacteria

Bacteria, singular bacterium, are a group of microscopic single-celled organisms that occupy a separate kingdom. They live in enormous numbers in almost every environment on Earth everywhere from deep-sea vents, to deep below Earth's surface, to the digestive tracts of humans. Bacterial cells differ from animal cells and plant cells in several ways. One fundamental difference is that bacterial cells lack intracellular organelles, such as mitochondria, chloroplasts (present only in plant cells, not in animal cells), and a nucleus, which are present in both animal cells and plant cells. Bacteria do not have a nucleus, but, instead, generally have a single chromosome: a piece of circular, double-stranded DNA located in an area of the cell called the nucleoid, or more appropriately plasmid (Figure 2).

Figure 2 Bacteria cell, animal cell, and plant cell, courtesy Encyclopaedia Britannica

Bacteria lack a membrane-bound nucleus and other internal structures. Therefore, they rank among the unicellular life-forms called prokaryotes. Prokaryotes are the dominant living creatures on Earth. They have been present for perhaps three-quarters of Earth history and have adapted to almost all available ecological habitats. As a group, they display exceedingly diverse metabolic capabilities. They can use almost any organic compound, and some inorganic compounds, as a food source.

Some bacteria can cause diseases in humans, animals, or plants. Still, most are harmless and are beneficial ecological agents whose metabolic activities sustain higher life-forms. Humans carry more bacteria in their gut than cells in their body. Some can cause diseases, but many serve useful functions. For example, good bacteria in humans prevent infections, provide micronutrients, help in the development of immunity. Other bacteria are symbionts of plants and invertebrates, where they carry out essential functions for the host, such as nitrogen fixation and cellulose degradation. Without prokaryotes, the soil would not be fertile, and dead organic material would decay much more slowly. Some bacteria are widely used in the preparation of foods, chemicals, and antibiotics. Studies of the relationships between different groups of bacteria continue to yield new insights into the origin of life on Earth and mechanisms of evolution (Rogers, 2019).

Individual bacteria can assume one of three basic shapes: spherical (coccus), rod-like (bacillus), or curved (vibrio, spirillum, or spirochete).

Bacteria are the smallest living entities. An average-size bacterium—such as the rod-shaped Ecoli, a typical inhabitant of the intestinal tract of humans and animals—is about 2 μm (μm = micrometre = 10-6 metre, or one-millionth of a metre) long and 0.5 μm in diameter. The spherical cells of Staphylococcus aureus are up to 1 μm in diameter. A few bacterial types are even smaller. Thus, bacteria are many times bigger in size than viruses.

Antoni van Leeuwenhoek discovered bacteria, in 1676, using a single-lens microscope of his own design. The pathogenic bacteria cause significant human death and disease. They cause infections such as tetanus, typhoid fever, diphtheria, syphilis, cholera, foodborne illness, leprosy, and tuberculosis. Bacterial infections may be treated with antibiotics (Wikipedia, Bacteria, 2020).

Sir Alexander Fleming, a Scottish biologist, defined new horizons for modern antibiotics with his discovery of the antibiotic substance penicillin (1928). The discovery of penicillin (from the fungus Penicillium notatum), and subsequent discoveries of other antibiotics and anti-bacterial drugs perfected the treatment of bacterial infections such as syphilis, gangrene, and tuberculosis (Explorable, 2020).

 Parasites

Parasites are organisms that live in or on an organism of another species (its host) and benefits by deriving nutrients at the other's expense. Parasites vary in size widely. Around 70 per cent parasites are not visible to the human eye, such as the malarial parasite. Still, some worm parasites can reach over 30 m in length.

Human parasites include various protozoa and worms that may infect humans that cause parasitic diseases. Human parasites are divided into endoparasites, which cause infections inside the body, and ectoparasites, which cause infection superficially within the skin. Malaria, kala-azar, amoebiasis, and giardiasis are some of the common diseases caused by endoparasites.  Louse, tick, flea, mosquito, bed bug, mite, are ectoparasites.

Generally, the discovery of parasites in ancient humans relies on the study of faeces and other fossilized material. The earliest known parasite in a human was eggs of the lung fluke found in fossilized faeces in northern Chile and is estimated to be from around 5900 BC. The first written records of parasites date from 3000 to 400 BC in Egyptian papyrus records. They identify parasites such as roundworms, Guinea worms, threadworms, and some tapeworms of unknown varieties. In ancient Greece, Hippocrates and Aristotle documented several parasites in the collection of works Corpus Hippocraticus (Wikipedia, Human parasite, 2020).

Malaria has ravaged the population of many countries and killed millions of humans. Malaria parasites were discovered in 1880 in France. Seven years later, in India, Sir Ronald Ross discovered that mosquitoes transmit the malaria parasite. For this discovery, he received his Nobel Prize in 1902 (Archivist, London School of Hygiene & Tropical Medicine, 2015).

Fungi and algae

Fungi, singular fungus, are plantlike organisms lacking chlorophyll, such as mushrooms, moulds, yeasts, and mildews. Modern biologists tend to place fungi in their own kingdom, not in the plant kingdom because they get their nutrients from other living things (or from the remains of living things that have died) rather than from photosynthesis.

Common diseases caused by fungi are: (a) skin diseases such as ringworm and athletes foot, (b) inflammation of mucous membranes like mouth, tongue, and vagina (commonly known as thrush), (c) ear infection like otomycosis, and (d) eye diseases like corneal infection called fungal keratitis. Fungal infection is killed naturally with home remedies like antifungal ointments, coconut oil, apple cider vinegar, and borax. Fungi can also cause serious life-threatening infections in immune-compromised patients.

Algae, singular alga, are in a different group of organism than fungi and belong to their own kingdom. Algae do not directly infect humans, but toxins produced by algae can cause diseases—for example, cell fish poisoning or some drinking water poisoning. Algae may cause the following diseases in humans: auto-immune diseases such as multiple sclerosis (MS), lupus, rheumatoid arthritis, and amnesic shellfish poisoning.

 Acknowledgement

The author is grateful to his friend and next-door neighbour, Dr Susanta Ghosh, PhD, FRCP, a distinguished haematologist, for reviewing this article. 

References

Archivist, London School of Hygiene & Tropical Medicine. (2015, 09 16). Ross and the Discovery that Mosquitoes Transmit Malaria Parasites. Retrieved from Centers for Disease Control and Prevention: https://www.cdc.gov/malaria/about/history/ross.html

Bing.com/images. (n.d.). Images of SARS-CoV-2. Retrieved from https://www.bing.com/images/search?q=sars-cov-2+size+microns&qpvt=sars-cov-2+size+microns&form=IGRE&first=1&scenario=ImageBasicHover

CMM International. (2017, 06 23). The Launch of the World's First Microsphere Nanoscope. Retrieved from CMM International Magazine: http://www.cmmmagazine.com/metrology/the-launch-of-the-world-s-first-microsphere-nanoscope/

Diagnosis, R. (2015, 08 13). Treatment of viral diseases. Retrieved from Right Diagnosis: https://www.rightdiagnosis.com/v/viral/treatments.htm

Explorable. (2020). History of Antibiotics. Retrieved from Explorable: https://explorable.com/history-of-antibiotics#:~:text=Sir%20Alexander%20Fleming%2C%20a%20Scottish%20biologist%2C%20defined%20new,bacterial%20infections%20such%20as%2C%20syphilis%2C%20gangrene%20and%20tuberculosis.

Goldsmith, C. S. (2009). Modern Uses of Electron Microscopy for Detection of Viruses. Retrieved from Clinical Microbiology Reviews - American Society of Microbiology: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2772359/

Nouri, A. (2011, 07 05). The discovery of bacteria. Retrieved from AAAS: https://www.aaas.org/discovery-bacteria

Prasad S, P. V. (2020). Transmission electron microscopy imaging of SARS-CoV-2. Retrieved from IJMR Indian Journal of Medical Research: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7224615/

Rogers, K. (2019, 06 19). Bacteria. Retrieved from Encyclopaedia Brtannnica: https://www.britannica.com/science/bacteria/The-cell-envelope

Wagner, R. R. (2020, 08 18). Virus. Retrieved from Britannica: https://www.britannica.com/science/virus/Malignant-transformation

Wikipedia. (2020, 08 26). Bacteria. Retrieved from Wikipedia: https://en.wikipedia.org/wiki/Bacteria

Wikipedia. (2020, 05 13). Human parasite. Retrieved from Wikipedia: https://en.wikipedia.org/wiki/Human_parasite#:~:text=Generally%2C%20the%20discovery%20of%20parasites%20in%20ancient%20humans,is%20estimated%20to%20be%20from%20around%205900%20BC.

World Health Organization. (2020). Naming the coronavirus disease (COVID-19) and the virus that causes it. Retrieved from World Health Organization: https://www.who.int/emergencies/diseases/novel-coronavirus-2019/technical-guidance/naming-the-coronavirus-disease-(covid-2019)-and-the-virus-that-causes-it

 

























Comments

  1. Shiladitya SenguptaJuly 31, 2024 at 9:52 AM

    Really an interesting and informative blog!

    ReplyDelete

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